Artículos científicos año 2008

Isolation and characterization of dental pulp stem cells from a supernumerary tooth. AH, Chen YK, Lin LM, Shieh TY, Chan AW. J Oral Pathol Med. 2008 Oct;37(9):571-4.

Abstract

BACKGROUND:

Dental pulp stem cells (DPSCs) were primarily derived from the pulp tissues of primary incisors and permanent third molar teeth, whereas no report to our knowledge has yet been documented on deriving DPSCs from the other tooth types. The aim of this study is to present a novel approach of harvesting stem cells from a super numerary tooth (a mesiodens).

MATERIALS AND METHODS:

The pulp tissues from a mesiodens of a 20-year-old healthy male patient and the left lower deciduous canine of a healthy 10-year-old boy (the positive control) were extracted and cultured for DPSCs, which were examined with stem cells (Oct-4, Nanog and Rex-1) and differentiation (Osteonectin and Nestin) markers. Furthermore, DPSCs were directionally differentiated to osteogenic and adipogenic cell lineages.

RESULTS:

Dental pulp stem cells derived from the mesiodens were capable of differentiating into adipogenic and osteogenic lineages. The mesioden's DPSCs also expressed stem cell and differentiation markers, which suggested their stem cell origin and differentiation capability. All the afore mentioned results for the mesiodens were consistent with those of the DPSCs derived from the positive control.

CONCLUSION:

We have demonstrated the feasibility of deriving DPSCs from a usually discarded tissue such as a supernumerary tooth.

http://www.ncbi.nlm.nih.gov/pubmed/18331285

Collection, cryopreservation, and characterization of human dental pulp derived mesenchymal stem cells for banking and clinical use. Perry BC, Zhou D, Wu X, Yang FC, Byers MA, Chu TM, Hockema JJ, Woods EJ, Goebel WS. Tissue Eng Part C Methods. 2008 Jun;14(2):149-56.

Abstract

Recent studies have shown that mesenchymal stem cells (MSC) with the potential for cell-mediated therapies and tissue engineering applications can be isolated from extracted dental tissues. Here, we investigated the collection, processing, and cryobiological characteristics of MSC from human teeth processed under current good tissue practices (cGTP). Viable dental pulp-derived MSC (DPSC) cultures were isolated from 31 of 40 teeth examined. Of eight DPSC cultures examined more thoroughly, all expressed appropriate cell surface markers and underwent osteogenic, adipogenic, and chondrogenic differentiation in appropriate differentiation medium, thus meeting criteria to be called MSC. Viable DPSC were obtained up to 120 h post extraction. Efficient recovery of DPSC from cryopreserved intact teeth and second-passage DPSC cultures was achieved. These studies indicate that DPSC isolation is feasible for at least 5 days after tooth extraction, and imply that processing immediately after extraction may not be required for successful banking of DPSC. Further, the recovery of viable DPSC after cryopreservation of intact teeth suggests that minimal processing may be needed for the banking of samples with no immediate plans for expansion and use. These initial studies will facilitate the development of future cGTP protocols for the clinical banking of MSC.

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2757034/